Coding
eGFP+His

Part:BBa_K193202:Design

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)


EGFP(enhanced Green Fluorescent Protein) with His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 732
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 732
    Illegal NheI site found at 750
    Illegal NotI site found at 762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 732
    Illegal BamHI site found at 528
    Illegal XhoI site found at 429
    Illegal XhoI site found at 771
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 732
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The gene sequence was obtained from clontech.

References